Coding

Part:BBa_K4632016

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)


T4-holin

Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.

What have we done? (SCAU-China 2023)

Codon was optimized for Escherichia coli expression. (We make contribution to the part BBa_K112805[1])

In order to reduce interference with the expression of T4 endolysin, we added a 20bp random sequence after T4 holin. We successfully characterized its performance in E. coli TOP10. For more detailed characterization results, please refer to our composite part BBa_K4632024 [2]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 256
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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